Fig. 2
HCQ enhances the sensitivity of lung cancer cells to chemotherapeutic drugs. a A549 cells were treated with different concentrations of HCQ for 24 h. The cytotoxicity of HCQ was detected by MTT. b A549 cells were pre-treated with PBS or 5 μM HCQ, followed by DOX (0.5 μg/mL) treatment for 24 h. Cell viability was detected by MTT analysis. c Lewis cells were pre-treated with PBS or HCQ, followed by DOX treatment for 24 h. Cell viability was detected by MTT analysis. d, e, and f A549 cells were pre-treated with PBS or HCQ, followed by 1 μg/mL MMC, 0.5 μg/mL PTX or 0.5 μg/mL Cis treatment for 24 h. Cell viability was detected by MTT analysis. g A549 cells were treated with 1 μM NH4Cl or 1 μM Met, followed by DOX treatment for 24 h. Cell viability was detected by MTT analysis. h A549/adr cells were pre-treated with PBS or HCQ, followed by DOX (5 μg/ml) treatment for 24 h. Cell viability was detected by MTT analysis. For all graphs, the error bars indicate the mean ± s.e.m., *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significant difference. The data shown are representative of three independent experiments