Fig. 4

HCQ-induced tumour suppression is CD8⁺ T cell-modulated. a A total of 2 × 10⁵ Lewis cells were intratracheally instilled into the lungs of nude mice. On day 10, the mice were instilled with 10 mg/kg HCQ and/or followed with 2 mg/kg DOX treatment by tail vein injection. On day 24, the mice were sacrificed for lung weight evaluation (n = 6). b Lewis-bearing C57BL/6 J mice were instilled with 10 mg/kg HCQ and/or followed with i.v. 2 mg/kg DOX treatment in the presence or absence of isotype or anti-CD8 neutralizing antibody. On day 24, the mice were sacrificed for lung weight evaluation (n = 5). c CD8⁺ T cell isolated from the spleens of C57BL/6 J mice were labelled with CFSE and stimulated with CD3CD28 beads in the presence or absence of HCQ. The histogram of CFSE was analysed with flow cytometry after 72 h (n = 3). d CD8⁺ T cell isolated from the spleens of C57BL/6 J mice were treated with or without HCQ, and CD69 and CD137 expression was analysed by flow cytometry (n = 3). e Normal C57BL/6 J mice were treated with PBS or HCQ. CD69 and CD137 expression in CD3⁺CD8⁺ splenocytes were analysed by flow cytometry (n = 3). f CD3⁻CD49b⁺ NK cells were sorted from the spleen and treated with or without HCQ for 3 days. The level of IFN-γ, TNF-α, IL-10 and MCP-1 in supernatant were detected by ELISA (n = 3). For all graphs, the error bars indicate the mean ± s.e.m., *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significant difference. The data shown are representative of three independent experiments