Fig. 2
Knockdown of HOTAIRM1 suppresses proliferation and induces apotosis of GBM cells and inhibits GBM xenograft tumorigenesis in vivo. a The qRT-PCR analysis of HOTAIRM1 RNA levels at 24 h after siHOTAIRM1 treatment, with the GAPDH gene as an internal control. The siControl was a scrambled sequence with no homology to any known gene. After treatment A172 cell with siHOTAIRM1 and siNC for 24 h, b cell growth curve was determined by CCK-8 assay at various time points (12 to 48 h); c representative flow cytometry cell cycle profiles and the plot showing changes in cell proliferation; d flow cytometry analysis showing cells apoptosis rate. e The qRT-PCR analysis of HOTAIRM1 RNA levels after transfection with lentivirus of shHOTAIRM1 or shControl with the GAPDH gene as an internal control, and representative images shower cells with GFP+. f After transfection with shHOTAIRM1 or shControl, A172 cell growth curve was determined by CCK-8 assay at a different time point (0.5 to 5 day). a-f Error bars represent the SEs of three independent experiments, *P < 0.05. g The anti-tumor effect of knockdown of HOTAIRM1 in vivo. shHOTAIRM1/U87 cells and shControl/U87 cells (1× 106 cells per mouse) were subcutaneously injected into nude mice. The mean tumor volumes were assessed at the indicated days, *P < 0.05, ** P < 0.01. h After 25 days, the mice were sacrificed. Representative nude mice from the shControl and shHOTAIRM1 groups