Fig. 4

HOTAIRM1 regulates HOXA1 RNA levels in GBM cells. a The exon composition of HOTAIRM1 and HOXA1 transcript and organization of the overlapping region of them. The RNA expression of HOXA1 was analyzed using qRT-PCR, b after transfection with shHOTAIRM1 or shControl in A172 cells; c in normal brain and glioma (WHO I - IV) tissues; d in normal brain, low grade glioma (WHO I and II), and high grade glioma (WHO III and IV) tissues, with the GAPDH gene as an internal control, *** P < 0.001. e The correlational analyses between HOTAIRM1 and HOXA1 RNA expression levels in GBM tissues. f qPT-PCR analysis of the RNA levels of the HOXA1 gene in HA cell, established and primary GBM cell lines. The qPCR was performed with the GAPDH gene as an internal control and error bars represent the SEs of three independent experiments, *P < 0.05, **P < 0.01. g Box plots depicting the mRNA levels of HOXA1 from 523 GBM patients and 10 normal brain tissues obtained from The Cancer Genome Atlas (TCGA), *** P < 0.001