Fig. 6

Knockdown of HOTAIRM1 induces CpG island methylation in the TSS region of the HOXA1 gene by increasing DNA demethyltransferases. a The location of CpG island in the TSS region of the HOXA1 gene and PCR segments of methylation specific PCR (MSP). The meCG levels in the HOXA1 gene were measured to analyze DNA methylation status b in HA cell, established and primary GBM cell lines; c after knockdown of HOTAIRM1 in A172 cells. d The model for transitioning from a hypomethylated status to a hypermethylated status in the TSS region of HOXA1gene. The green and black box mark the predicted promoter regions of the HOXA1 gene. e After knockdown of HOTAIRM1, ChIP analysis of DNA methyltransferase binding in the HOXA1 TSS region in A172 cells. Error bars represent the SE of three independent experiments, *P < 0.05