Fig. 5

E2F1 enhanced Nanog expression. a The Nanog gene promoter region. b Chromatin immunoprecipitation (ChIP) assay was performed to identify which region functioned as the effective binding site of Nanog promoter region. c The luciferase reporter plasmid having wild type or mutant sequences of Nanog promoter region (− 586 ~ − 576) were established. d The luciferase activity of wild type sequences and mutant of Nanog promoter region. e, f Nanog protein expression when transfected with enhanced E2F1 plasmid or E2F1 silencing