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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: GABA, glutamine, glutamate oxidation and succinic semialdehyde dehydrogenase expression in human gliomas

Fig. 1

Oxygen consumption, extracellular acidification rate and glutamine oxidation differences were measured by Seahorse in U87 MG, U251 wt, U373 MG glioma cell lines using D5030 medium. a Different oxygen consumption rates in the studied cell lines (pmol/min). b Extracellular acidification rates were measured in minimal medium (D5030) at basal rate and after glucose (10 mM) addition following oligomycin (2 μM) treatment. c Oxygen consumption rate curves in minimal medium (D5030), and after glutamine (2.5 mM) addition and using several metabolic inhibitors and modulators (oligomycin - 2 μM; 2,4-dinitrophenol - DNP - 100 μM; antimycin - 1 μM - and rotenone - 1 μM) *:p < 0.05. d Time-dependent growth of different glioma cell lines (U87 MG, U251 wt and U373 MG) at normal non-starving conditions. Cell proliferation was monitored by SRB test using 2.5 × 103 cells/100 μl at the starting point of the experiments

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