Fig. 2
IDH1 mutation (IDH1m) and the elevated 2-HG level related metabolic alterations in U251 glioma cells (oxygen consumption and metabolic enzyme expression differences). a Oxygen consumption rates in U251 wt, U251 IDH1m and 2-HG pre-treated (72 h, 4 mM) U251 wt cells (OCR was measured in D5030 medium by Seahorse). b Extracellular acidification data in minimal medium (D5030), and after glucose (10 mM) addition and using oligomycin (2 μM) thereafter (Seahorse measurements). c Alterations in the expression of different metabolic enzymes in IDH1m and 2-HG-treated (72 h, 4 mM) U251 glioma cells (representative Western blot results using anti-β-F1-ATPase - ATPB, anti-hexokinase 2 - HK2, anti-phosphofructokinase P - PFKP and β-actin). d Differences in proliferation of U251 wt, IDH1m and 2-HG pre-treated (72 h) wt cells. The relative proliferation was calculated related to U251 wt (100%), the growing capacity was monitored by SRB tests using 2.5 × 103 cells/100 μl at the starting point of the experiments; *:p < 0.05