Skip to main content
Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: GABA, glutamine, glutamate oxidation and succinic semialdehyde dehydrogenase expression in human gliomas

Fig. 4

Energy substrate oxidation differences in wt, IDH1m and 2-HG-treated U251 wt cells. a Oxygen consumption compared to basal respiration (%) after using different energy substrates: glucose (10 mM), glutamine (2.5 mM), citrate (5 mM), GABA (5 mM), acetate (10 mM), malate (10 mM), lactate (5 mM), glutamate (5 mM) in U251 wt and U251 IDH1m cell lines in D5030 medium by Seahorse measurements, *:p < 0.05 **:p < 0.01. b Effects of 2-HG on glutamine and GABA oxidation in U251 wt, IDH1m and 2-HG-treated wt cells (the oxidation was measured after with or without 2-HG (4 mM) pre-treatment (72 h) in D5030 by Seahorse measurements) *:p < 0.05, **:p < 0.01. c GABA metabolism related enzyme (GAT1 - GABA transporter 1; SSADH - succinic semialdehyde dehydrogenase) expressions; glutamine metabolism related enzyme expressions (glutaminase, ASCT2 - alanine, serine, cysteine-preferring transporter 2); and acetate consumption related ACSS2 (acetyl-CoA synthetase 2) enzyme expression in U251 wt, IDH1m and 2-HG (4 mM) treated cells (72 h) using Western blot analyses. d Different growing characteristics of U251 wt and U251 IDH1m cells using long-term (3-week-long) 2-HG (4 mM), GABA (5 mM) and combination treatments in DMEM high glucose. After 3-week long-term continuous treatment (intermittently passaging 2 or 3 times per week) 2.5 × 103 cells/100 μl were plated for 72 h and terminated by SRB tests. Data show the average of relative proliferations (untreated U251 wt – 100%) *: p < 0.05

Back to article page