Fig. 4

LAT2 binds to p-mTOR^Ser2448 to upregulate LDHB and activate glycolysis. a Western blotting showed that mTOR inhibition (RAD001) downregulated LDHB in MIA PaCa-2 and PANC-1 cells. b Immunoprecipitation was used to confirm that LAT2 could bind to p-mTOR^Ser2448 to activate mTOR. c Immunoprecipitation was used to confirm that p-mTOR^Ser2448 could bind to LDHB to upregulate the expression of LDHB. d, e Immunofluorescence was used to confirm that LAT2 could bind to p-mTOR^Ser2448 to upregulate LDHB; LAT2 was localized to the cytomembrane and cytoplasm; however, both p-mTOR and LDHB were localized to the cytoplasm