Fig. 2

The compound kushen injection inhibited AML cell proliferation. a, b Test the cell proliferation of four AML cells (a) or (b) human patient cells isolated from AML patients were treated with three doses of CKI at 28 μl/ml, 56 μl/ml, and 110 μl/ml for 48 h using CCK8 kits. c, d Test the cytotoxicity and cell pore-forming activity of three AML cell lines (c) and human patient cells (d) were treated with three doses of CKI at 28 μl/ml, 56 μl/ml, and 110 μl/ml for 48 h by LDH release assay. e The inhibition effects of CKI were examined in U937 cells at different doses for 48 h, and the half maximal inhibitory concentration (IC50) values was determined. f The cell death rate was tested by MTT assay. Haematopoietic stem cells (HSCs) and T cells were treated with 28 μl/ml CKI or 2.5 mg/ml Ara-c for 48 h. HLL, hyperleukocytic leukaemia. Bars represent ±SEM