Fig. 3

The variation in cell morphology observed with the optofluidics chips at the single-cell microfluidics level. a Optofluidics chip used for cell cytotoxic analysis. For AML cell lines, D1 = 7 μm and D2 = 35 μm. For AML patient cells, D1 = 5 μm and D2 = 30 μm. b The cell motion morphology of AML cells was observed after 110 μl/ml CKI treatment at the single-cell level on the microfluidics chip. AML: AML patient cells (PC) 2#. c Molm-13 GFP+ cells were treated with 110 μl/ml CKI on optofluidics chips and observed under a laser scanning confocal microscope. Upper line: pyroptosis. Bottom line: apoptosis. d Molm-13 GFP+ cells were treated with 110 μl/ml CKI for 48 h and observed under an OLYMPUS IX73 fluorescence microscope. Magnification of the objective lens: × 20. e The response time for AML cell death