Fig. 4

Cell apoptosis and cell cycle analysis in U937 cells. a Apoptosis was tested by flow cytometry after U937 cells were treated with 110 μl/ml CKI for 48 h. b The morphological apoptotic characteristics were observed by TEM after U937 cells were treated with 110 μl/ml CKI for 48 h. Magnification: × 2000. c Early apoptosis was determined by JC-1 assay after U937 cells were treated with 110 μl/ml CKI for 24 h. d Cell cycle was analysed by flow cytometry after U937 cells were treated with CKI for 48 h