Fig. 4

XIST targets miR-34a to negatively interact with miR-34a (a) KAT18 and FTC133 cells were transfected with miR-34a mimics or miR-34a inhibitor to achieve miR-34a expression, as confirmed using real-time PCR assays. b XIST expression was determined using real-time PCR assays. c miR-34a expression in response to XIST knockdown was determined using real-time PCR assays. d-e Luciferase reporter gene assays were performed to confirm the direct targeting of XIST to miR-34a. f Association of miR-34a and XIST with AGO2 in HEK293 cells. Detection of AGO2 and IgG using Immunoblotting assays. g RIP assay in HEK293 cells transfected with control miRNA (miR-NC) or miR-34a followed by real-time PCR to detect XIST associated with AGO2. The data are presented as mean ± SD of three independent experiments. **P < 0.01