Fig. 2
UBE2D1 promoted HCC growth in vitro and in vivo. a Confirmation for the overexpression and knockdown of UBE2D1 by western blot. The value of blotting score was showed in the bottom panel. b Plate clone formation assays in UBE2D1 overexpressed or underexpressed QSG-7701 and LM3 cells. The number of clones was showed in the right panel. c CCK-8 assays for cell proliferation in UBE2D1 overexpressed and silent LM3 and QSG-7701 cells. d Apoptosis markers determined by western blot to evaluate the anti-apoptosis role of UBE2D1 in LM3 and QSG-7701 cells. e Tumors from nude mice with 1 X 105 indicated cells injected subcutaneously in bilateral armpit. n = 8 for each group. And the tumor incidence and tumor volume for each group was showed in the right panel. *p < 0.05,**p < 0.01, ***p < 0.001