Fig. 3
P53 mediated the pro-tumor effect of UBE2D1. a p53 protein level determined by western blot in UBE2D1 overexpressed and underexpressed QSG-7701 cells. The quantitation of western blot from 3 independent experiments was showed in the right panel. b P53 western blot assays in UBE2D1 silent and negative control QSG-7701 cells after treated with MG132 for 12 h and 24 h. The quantitation of western blot from 3 independent experiments was showed in the right panel. c After immunoprecipitation with specific antibodies of MDM2, the purified proteins were detected by western blot in UBE2D1 silent QSG-7701 cells. Immunoprecipitation using mouse lgG antibody was performed as negative control. Input, the whole cell lysate. d CCK-8 assays in UBE2D1 underexpressed SMMC-7721, L02, Huh7 and Hep3B cells. e Protein levels of apoptosis markers in UBE2D1 underexpressed SMMC-7721, L02, Huh7 and Hep3B cells. f Tumors from nude mice with 1 X 105 indicated cells injected subcutaneously in bilateral armpit. n = 5 for each group. And the tumor incidence and tumor volume for each group was showed in the right panel. *p < 0.05,**p < 0.01