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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Mutated Von Hippel-Lindau-renal cell carcinoma (RCC) promotes patients specific natural killer (NK) cytotoxicity

Fig. 1

VHL-MUT-RCC patients display higher NK cytotoxicity toward A498, VHL-MUT RCC cell line. NK cell function was evaluated in whole blood through CD107a cell-surface expression and production of IFN-γ in response to ex vivo stimulation with K562, A498 and CAKI-1 cells. Degranulation (CD107a; A, B, C) and cytokine production (IFN-γ; D, E, F) were evaluated after gating on CD3CD56+ cells. Percent of degranulation was obtained subtracting the percent of degranulation occurring in the absence of target cells. CD107a+NK cells in 12 HD (A), 28 VHL-WT (B) and 23 VHL-MUT (C) RCC patients versus K562, CAKI-1 and A498 renal cell lines. Representative profiles of the percentages of CD107a+NK cells in HD (Ai) VHL-WT (Bi) and VHL-MUT (Ci) RCC patients. IFN-γ production was assayed by intracellular cytokine staining in 8 HD (D), 13 VHL-WT (E) and 14 VHL-MUT (F) RCC patients versus K562, CAKI-1 and A498 renal cell lines. Representative profiles of the percentages of IFN-γ+ NK cells in HD (Di) VHL-WT (Ei) and VHL-MUT (Fi) RCC patients. The results are presented mean ± SEM. Statistical significance was calculated by unpaired Student t test (p < 0.05)

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