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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Mutated Von Hippel-Lindau-renal cell carcinoma (RCC) promotes patients specific natural killer (NK) cytotoxicity

Fig. 4

Tumor-Tregs from VHL-WT-RCC patients are more suppressive than tumor-Tregs from VHL-MUT-RCC patients. A Percentage of tumor CD4+CD25hiFoxp3+cells (13 VHL-WT-RCCs vs 13 VHL-MUT-RCCs, p = 0.04). (Ai) Representative plots of Tregs from 1 VHL-WT-RCCs vs 1 VHL-MUT-RCCs. B Autologous CFSE-labeled CD4+CD25 T cells were co-cultured with CD4+CD25+ isolated from tumor of 6 VHL-WT and 4 VHL-MUT RCC patients. After 5 days of stimulation with Dynabeads Human T-Activator CD3/CD28, CFSE+CD4+ T cells were analyzed for their proliferation by CFSE dilution. C IFN-γ-tumor-Treg dependent ELISA from 3 VHL-WT vs 3 VHL-MUT RCC patients. D Measurement of TGF-β1 mRNA expression in 6 VHL-WT vs 6 VHL-MUT RCC patients by real-time PCR in tumor and corresponding peritumoral tissues. Relative mRNA expression was calculated according to 2-ΔΔCt method. The results are presented mean ± SEM. Statistical significance was calculated by unpaired Student t test (p < 0.05)

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