Skip to main content
Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Co-occurring KRAS mutation/LKB1 loss in non-small cell lung cancer cells results in enhanced metabolic activity susceptible to caloric restriction: an in vitro integrated multilevel approach

Fig. 5

KS enhanced the glucose and glutamine metabolic flux. a Percentage of 13C incorporation into TCA cycle intermediates after addition of 13C-glucose and 13C-glutamine (steady state, 24 h of labelling) in NSCLC NCI-H1299 derived clones KS, K, S and WT, respectively. b Kinetic analysis of glucose incorporation in presence of KS, K, S and WT genetic backgrounds in NSCLC NCI-H1299 incubated with 13C-glucose after 1, 2, 4, 8 h. c Kinetic analysis of glutamine incorporation in presence of KS, K, S and WT backgrounds in NSCLC NCI-H1299 incubated with 13C-glutamine after 1, 2, 4, 8 h. M + 2, + 3 or + 4 labeled compounds indicate molecules of those compounds that contain 2, 3 or 4 13C atoms, respectively. d Ammonia release (nM) in conditioned medium in presence of KS, K, S and WT backgrounds in NSCLC NCI-H1299 after 48 h from cell seeding. p-values were calculated using one-way ANOVA test and Tukey Kramer post-test for multiple comparisons (GraphPad Prism, V7.02). Significant differences are marked as * vs WT; # KSvsK; § KSvsS; @ KvsS The number of symbols refer to the level of significance: one p < 0.05; two p < 0.01, three p < 0.001

Back to article page