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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: RETRACTED ARTICLE: MNAT1 is overexpressed in colorectal cancer and mediates p53 ubiquitin-degradation to promote colorectal cancer malignance

Fig. 3

MNAT1 decreases p53 expression. A, HCT116 cells were transfected with Flag-MNAT1 (a), and DLD1 cells were transfected with pSIN-MNAT1 (b), p53 expressions the tranfected cells were detected using Western-blotting. B, PT53 mRNA expressions in the transfected cells were detected using Real-time PCR. C, HEK293T cells were cotransfected with HA-p53 and Flag-MNAT1 at various concentrations, Flag and HA expressions were detected (a). Abundances of Flag and HA were analyzed (b). D, LoVo cells were transfected with shsramble, shMNAT1#1 and #2, and then p53 and MNAT1 expressions in the transfected cells were detected using Western-blotting. E. LoVo cells were treated with COX at the indicated concentrations, and then MNAT1 and p53 expressions were detected. F, HEK293T cells were transfected with pSIN-MNAT1 or blank vector, and then treated with DOX. MNAT1 and p53 expressions were detected using Western-blotting. G, HEK293T cells transfected with pSIN-MNAT1 were treated with DOX and/or MG132, and then MNAT1 and p53 were detected. DOX, Doxorubicin; *, p < 0.05

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