Fig. 4

MNAT1 interacts with p53. A, the lysates of LoVo cells were immunoprecipitated using anti-IgG, anti-MNAT1 or anti-p53 antibodies, and immunoprecipitation products were analyzed by Western-blotting with the indicated antibody. B, HEK293T cells were cotransfected with Flag-MNAT1 and HA-p53. Flag or HA was immunoprecipitated in the transfected cells, and the immunocomplexes were detected using Western-blotting with HA- or Flag-antibody. C, The various construct plasmids of TP53 were constructed using PCR (a). HEK293T cells were cotransfected with Flag-MNAT1 and the indicated plasmids of TP53. Immunoprecipitation was performed using anti-HA antibody, and the immunoprecipitated products were detected using Western-blotting with anti-Flag antibody. D, The plasmids containing various MNAT1 gene segments were constructed (a). HEK293T cells were cotransfected with HA-TP53 and the indicated plasmids of Flag-MNAT1. Immunoprecipitation was performed using anti-Flag antibody, and the immunoprecipitated products were detected using Western-blotting with anti-HA antibody. FL, full length p53; IP, immunoprecipitation; WCL, whole cell lysate