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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: MNAT1 is overexpressed in colorectal cancer and mediates p53 ubiquitin-degradation to promote colorectal cancer malignance

Fig. 5

MNAT1 promotes p53 ubiquitin-mediated degradation. A, HEK293T cells were cotransfected with HA-Ub, Flag-p53, and v5-MNAT1. Flag was immunoprecipitated in the transfected cells, and p53 was detected using Western-blotting (a), and p53 life-time was calculated (b). B, LoVo cells were cotransfected with HA-Ub, Flag-p53 and pLVX-shMNAT1#1 or pLVX-shMNAT1#2, respectively. The transfected cells were immunoprecipitated with Flag antibody, and p53 in the immunocomplexes were detected. C, HCT116 cells were cotransfected with pLVX-shMNAT1#1 or pLVX-shMNAT1#2, respectively. The transfected cells were immunoprecipitated with p53 antibody, and then ubiquitin in the immunocomplexes was detected. D, HEK293 cells were transfected with pSIN-MNAT1, and p53 in the transfected cells was immunoprecipitated. Ubiquitin in the immunocomplexes was detected. E, HCT116 cells were cotransfected with pLVX-shMNAT1#1 and #2. p53 in the transfeccted cells was immunoprecipitated, and its ubiquitination was detected. IP, immunoprecipitation; Ub, ubiquitin; WCL, whole cell lysate

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