Fig. 1

Endogenous levels of miR-296-5p inversely correlated with aggressive traits in HCC. (a) A heatmap of 15 differentially expressed miRNAs between HCC cell lines with high and low metastatic potentials. Columns represent 11 high and 7 low metastatic HCC cell lines. Colorgram depicts relative high (red) and low (blue) expression levels of miRNAs. (b) Representative images (left) and expression levels (right) of endogenous miR-296-5p in tumor tissues and paired adjacent normal liver tissues of 89 HCC patients by miRNA in situ hybridization. Magnification: 100× (above), 400× (below). (c) The OS and DFS in high and low miR-296-5p expressed HCC patients. (d) Representative images of the EMT-associated markers in HCC tissues with high or low level of miR-296-5p by IHC staining (left). Relationship between miR-296-5p levels and EMT-associated markers expression (right). magnification 100×. ***p < 0.001