Fig. 2

miR-296-5p inhibits EMT process and cell metastasis in vitro. (a) EMT-associated morphological changes in miR-296-5p-KD Huh7, miR-296-5p-OE MHCC97H cells and their negative control cells. Magnification 200×. (b) Western-blot analysis of protein levels of EMT markers and transcription factors in miR-296-5p-KD Huh7, miR-296-5p-OE MHCC97H cells and their negative control cells. GAPDH served as an internal control. (c) Immunofluorescence images of E-cadherin and Vimentin in miR-296-5p-KD Huh7, miR-296-5p-OE MHCC97H cells and their negative control cells. Magnification 200× (d) Transwell assays (left) and statistical results (right) of cellular migration and invasion at 48 h. magnification 100×. (e) Representative wound-healing images (left) and statistical results (right) of indicated cells at 0 and 48 h. magnification 100×. **p < 0.01, ***p < 0.001