Fig. 4

miR-296-5p inhibits tumor growth and lung metastasis in vivo. Xenograft tumor model was established in nude mice using miR-296-5p-OE MHCC97H cells and their negative control cells. (n = 6 for each group). (a) Images (left) and volumes (right) of primary liver tumors in recipient mice after 7 weeks. (b) The expression of NRG1, EMT markers (E-cadherin and Vimentin) in xenograft tumors with indicated cells analyzed by immunohistochemical analysis. Magnification 200×. (c) Hematoxylin and eosin (H&E) staining of metastatic liver nodules (left) and the percentage of mice with or without metastatic nodules in the livers (right). (d) Representative pictures for lung metastasis (left) and the percentage of mice with or without metastatic nodules in the lungs (right). magnification × 100 (left); 400× (right). **p < 0.01