Fig. 3

Downregulation of DMTN enhances the activity of the Rac1 signaling pathway by relieving binding to the ARHGEF2 protein. a, GSEA analysis of the “Rac1 signaling pathways” gene set in the low versus high expression group of DMTN in CRC using the GEO data. b, Western blot analysis of the expression of Rac1-GTP in the CRC cell lines with DMTN overexpression or knockdown. c, Immunofluorescence detection of the colocalization between DMTN with ARHGEF2 in CRC-immortalized cells (FHC) and CRC cells (SW480 and HT29). d, Coimmunoprecipitation (Co-IP) analysis of the protein interaction between DMTN with ARHGEF2 in CRC-immortalized cells (FHC). e, GST-pull-down detection of the interaction between the DMTN core domain (with an HA tag) and the ARHGEF2 DH domain (with a flag tag). f, Western blot analysis of the activity of Rac1 in the SW480 cells cotransfected with the ARHGEF2 and DMTN vectors