Fig. 1

ARS and DHA reverted L-929-CAFs from activated to inactivated state. L-929-CAFs were exposed to 50 μM of ART, ARM, ARS or DHA for 24 h. (a) Chemical structure of ART, ARM, ARS and DHA. (b) Effect of ART, ARM, ARS and DHA on cell viability by CCK-8 cell counting assay. (c-e) Immunofluorescence staining assay for α-SMA, fibronectin and vimentin were performed to test the effect of ART, ARM, ARS and DHA on CAFs related biomarker proteins (image magnification: 400×). (f) The expression of MMP-9, MMP-2, MMP-14, FAP, fibronectin, vimentin, α-SMA and S100A4 proteins in the cells were analyzed by western blot using specific antibodies. Each experiment was performed at least three times. Data are presented as mean ± SD. *p < 0.05 compared with CM-induced group; **p < 0.01 compared with CM-induced group