Fig. 6

ARS and DHA inactivated CAFs through suppressing TGF-β signaling in vivo. (a) TUNEL, sirius red staining and immunohistochemical detection of Ki67, α-SMA and vimentin protein levels on CAFs related biomarker in orthotopic site (image magnification: 400×). (b) The expression of MMP-9, fibronectin, vimentin, and α-SMA proteins were analyzed in orthotopic site by western blot using specific antibodies. A GAPDH antibody was used to check equivalent protein loading. (c) The effect of paclitaxel, ART, ARM, ARS and DHA on TGF-β1 content of tumor tissue. (d) Immunohistochemical detection of TGF-β1 protein levels in orthotopic site (image magnification: 400×). (e) The expression of p-Smad3 (Ser423/425), Smad3 and TGF-β1 proteins were analyzed in orthotopic site by western blot using specific antibodies. Each experiment was performed at least three times. Data are presented as mean ± SD. *p < 0.05 compared with 4 T1 + L-929 group; **p < 0.01 compared with 4 T1 + L-929 group