Fig. 8From: Tspan8 and Tspan8/CD151 knockout mice unravel the contribution of tumor and host exosomes to tumor progressionTspan8, CD151 and angiogenesis in ko mice and ko MCA tumors. Dispersed lung cells were cultured for 3wk-4wk to enrich and expand EC. EC were cocultured with wt-sExo or wt- and ko-TEX for 48 h–72 h. a EC flow-cytometry analysis of tetraspanins, EC markers, chemokines and angiogenic receptors including representative examples and (b) of MCA angiogenic receptor; a,b mean % stained cells (3 assays), significant differences between wt- and ko-EC or -MCA cells: *, significant differences by coculture with sExo: s (grey) or TEX: s (black); c-e confocal microscopy of wt-, Tspan8ko-, and CD151ko-EC cultured in the absence or presence of wt-sExo or -TEX and stained with anti-Tspan8 or anti-CD151 and counterstained with (c) anti-CD184, d anti-EphB4, e anti-VEGFR2, single staining and overlays are shown (scale bar: 10 μm); f untreated and wt-TEX-treated wt- and ko-MCA lysates were precipitated with anti-VEGFR2, -VEGFR3, -FGFR3 and EphA4. Dissolved precipitates were blotted with anti-Tspan8 or anti-CD151. Representative WB examples are shown. Ko-EC express CD31, CD34, OPN, VEGFR2, and EphA4 at a reduced level; in CD151ko-EC additionally CD106 and CD62P expression is reduced. Wt-sExo promote OPN, VEGFR2 and EphA4 expression in ko-EC; wt-TEX activate EC marker, OPN, SDF1, VEGFR2, EphA4 and CD184 expression in wt- and ko-EC. In ko-MCA cells, wt-sExo and -TEX support VEGFR2, VEGFR3 and EphA4 expression. Upregulated expression, accompanied by colocalization and coimmunoprecipitation, might well account for Tspan8- and CD151-TEX promoted EC activationBack to article page