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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Hepatocellular carcinoma-derived exosomal miRNA-21 contributes to tumor progression by converting hepatocyte stellate cells to cancer-associated fibroblasts

Fig. 4

Exosomal miRNA-21 is characteristically secreted by liver cancer cells and mediates HSCs activation. a Expression of exosomal miRNAs from different cancer cells was presented. Wound-healing assay (b), migration assay (c), Western blotting assay (d), immunofluorescence assay (e) and CCK-8 assay (f) of HSCs transfected miRNA-21-mimic or normal control. Representative images were shown. g qPCR of miRNA-21 in HCC-derived treated with miRNA-21 inhibitor exosomes. h Western blotting assay of HSCs transfected miRNA-21 inhibitor. Experiments were performed at least in triplicate, and results are shown as mean ± s.d. Student’s t-test was used to analyze the data (NS, not significant; *p < 0.05; **p < 0.01; ***p < 0.001)

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