Fig. 1

Relative miR-10b-3p expression levels in ESCC tissues and serum and their clinical significance. a Expression levels of miR-10b-3p in 93 paired ESCC and corresponding noncancerous tissues as measured by in situ hybridization. b Kaplan–Meier overall survival curves of high and low miR-10b-3p expression in 93 cases of ESCC. c Quantitation of miR-10b-3p was performed using qRT-PCR in 102 paired ESCC (T) and corresponding normal tissues (N). The fold changes were calculated by relative quantification (2^-∆Ct, U6 as the internal control). d-e, miR-10b-3p expression was detected in lymph node metastasis (d) and different clinical stages (e) of ESCC. f Kaplan-Meier curves depicting overall survival according to the expression of miR-10b-3p as validation. g The expression level of serum miR-10b-3p in 92 ESCC patients and 50 healthy controls was measured by qRT-PCR and normalized to U6. h-i, miR-10b-3p expression was detected in lymph node metastasis (h) and different clinical stages (i). j Receiver operating characteristic (ROC) curve analysis of the miR-10b-3p assay ratio for detecting ESCC patients. Each experiment was performed in triplicate