Fig. 2

DNA methylation status of miR-10b-3p. a Genomic structure and distribution of miR-10b-3p CpG dinucleotides over the transcription start site (TSS). b The positions and orientation of the MassARRAY primers are indicated by horizontal black bars. Each column represents a sample. Each row displays the clustering of CpG units, which are a single CpG site or a combination of CpG sites. The color gradient between blue and yellow indicates methylation of each miR-10b-3p unit in each sample ranging from 0 to 100%. Gray represents technically inadequate or missing data. c Gene location, amplicon size, and place of CpG sites in the amplicon. Methylation profile of CpG sites for the miR-10b-3p gene. The color of the circles is related to the percentage of methylation at each CpG site. Boxes indicate the different methylation patterns between 18 ESCC samples and corresponding normal tissues. d Evaluation of CpG methylation within the miR-10b-3p promoter. The distribution of 13 analyzed CpG units within miR-10b-3p. e DNA methylation levels of the miR-10b-3p promoter region in 5-Aza-CdR-treated ESCC cells as detected by BSP assay. f Quantitation of the miR-10b-3p level after treatment with 5-Aza-CdR in KYSE150 and KYSE450 cell lines. Each experiment was performed in triplicate. Data are presented as the mean value ± SD. ^●, P < 0.05; ^●●, P < 0.01; ^◆, P > 0.05