Fig. 4

Repression of miR-10b-3p expression significantly inhibited cell growth, colony formation, and migration in ESCC cells. a Quantitation of the miR-10b-3p level after transfection of the miR-10b-3p inhibitor in KYSE30 and KYSE510 cell lines. b-c The cell growth curve was measured by MTS after transfection of the miR-10b-3p inhibitor in KYSE30 and KYSE510 cell lines, and the OD 570 was normalized to the star point (0 h). d Representative images and quantitation of colony formation were performed after transfection of the miR-10b-3p inhibitor in KYSE30 and KYSE510 cell lines. e Representative images and quantitation of the transwell assay were performed after transfection of the miR-10b-3p inhibitor in the KYSE30 and KYSE510 cell lines. f miR-10b-3p inhibitor induced apoptosis. Each experiment was performed in triplicate. Data are presented as the mean value ± SD. ^●, P < 0.05; ^●●, P < 0.01