Fig. 1

RBMS2 expression in breast cancer tissues. a Comparison of RBMS2 transcript expression in tumor and normal tissues of breast cancer in the Cancer Genome Atlas (TCGA) database (p < 0.01). The expression values were log2-transformed. b Kaplan-Meier survival curves of RFS based on RBMS2 expression in breast cancer using Kaplan-Meier plotter. The cut-off value was the median expression of RBMS2. c RBMS2 mRNA expression in 46 pairs of breast cancer and adjacent tissues. d Average expression level of RBMS2 mRNA in 46 pairs of human breast cancer tissues and adjacent normal breast tissues. Adjacent breast tissues had higher expression of RBMS2 than breast cancer tissues (***p < 0.001). e qRT-PCR (upper) and western blot (lower) of RBMS2 in breast cancer cell lines and in normal breast cell lines MCF-10A. The average level of mRNA in MCF-10A was set as 1. f RBMS2 mRNA in breast cancer cell lines and in normal breast cell lines hTERT-HME1 in the Human Protein Atlas database. Expression quantification was shown as TPM (Transcripts Per Million). h Representative IHC stains of RBMS2 in normal and tumor breast tissues in the Human Protein Atlas database. Scale bars indicated 250 μm