Fig. 2
From: RBMS2 inhibits the proliferation by stabilizing P21 mRNA in breast cancer
Construction of RBMS2 overexpression and knockdown cell lines. Western blot (a) and qRT-PCR (b) were used to confirm RBMS2 overexpression after tansfection with lentivirus in MCF-7 and SUM-1315 cell lines. The upper band of RBMS2 is the specific band in western blot. NC, control; RBMS2, lentivirus overexpressing RBMS2; The growth of cells was measured using CCK8 assays in RBMS2 overexpressing MCF-7 (c) and SUM-1315 (d) cell lines. Western blot (e) and qRT-PCR (f) were used to confirm RBMS2 knockdown after transfection with lentivirus in MCF-7 and SUM-1315 cell lines. SCR, control; shRBMS2, lentivirus knocking down RBMS2. The growth of cells was measured using CCK8 assays in RBMS2 knockdown MCF-7 (g) and SUM-1315 (h) cell lines