Fig. 3

Effect of RBMS2 on proliferation and growth of breast cancer cell lines MCF-7 and SUM-1315 in vitro andin vivo. The growth of cells was measured using colony formation assays in RBMS2-overexpressing MCF-7 and SUM-1315 cell lines. Representative photographs (upper) and quantification (lower) were shown.(**p < 0.01,***p < 0.001). The growth of cells was measured using colony formation assays in RBMS2-knockdown MCF-7 and SUM-1315 cell lines. Representative photographs (upper) and quantification (lower) were shown (**p < 0.01,***p < 0.001). Cell cycle progression was measured using flow cytometry in RBMS2-overexpression MCF-7 (c) and SUM-1315 (d) cell lines. Representative photographs (upper) and quantification (lower) were shown. (*p < 0.05) (e) Tumor volume in RBMS2-overexpressing SUM-1315 compared with control in nude mice in different time points (f) Tumor weight in RBMS2-overexpressing SUM-1315 compared with control in nude mice at the end point. Quantifiaction (upper) and representative photographs (lower) were shown