Fig. 4

Differential expression genes and pathways regulated by RBMS2. a Total 285 genes were differentially identified from mRNA sequencing. Among them, 177 genes (red) were up regulated and 108 genes (blue) were down regulated. b Gene Set Enrichment Analysis (GSEA) of differentially expressed genes. c Heatmap of differentially expressed genes from mRNA sequencing (upper). Heatmap of expression of CDKN1A and RBMS2 from mRNA sequencing (down).Sky blue indicated RBMS2 overexpression cell lines and red indicates control cell lines. The MCF7 cells were transfected with lentivirus to overexpress RBMS2 (d) or knock down RBMS2 (e). The P21 expression was obviously increased after upregulating RBMS2 at both protein and mRNA levels (d). The P21 expression was obviously decreased after knocking down RBMS2 at both protein and mRNA levels (e). Western blot (left) and mRNA (right) photographs were shown. The SUM-1315 cells were transfected with lentivirus to overexpress RBMS2 (f) or knock down RBMS2 (g). The P21 expression was obviously increased after upregulating RBMS2 at both protein and mRNA levels (f). The P21 expression was obviously decreased after knocking down RBMS2 at both protein and mRNA levels (g). Western blot (left) and mRNA (right) photographs were shown