Fig. 2

Tetraspanin 1 (TSPAN1) promotes CCA cell proliferation and tumorigenicity in vitro and in vivo. (a) Proliferation rate was analyzed by CCK-8 assay of indicated CCA cells (Con, LV-TSPAN1, shCon, and LV-shTSPAN1). (b) Representative images of colony formation assays are shown in the upper panels; the number of foci was counted as shown in the panels below. (c) TSPAN1 upregulation enhanced CCLP1 cell xenograft tumor growth in nude mice while TSPAN1 knockdown reduced HCCC9810 growth. (d) Immunohistochemical (IHC) staining was used to detect Ki-67 expression in xenograft tissues (200× magnification), scale bars = 50 μm. Data are means ± SD of three independent experiments. *p < 0.05 and **p < 0.01