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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Dysregulation of miR-6868-5p/FOXM1 circuit contributes to colorectal cancer angiogenesis

Fig. 5

miR-6868-5p/FOXM1 axis regulates CRC angiogenesis via IL-8. a qRT-PCR analysis of the expression of angiogenic factors in HCT8 and HCT116 cells transfected with control vector or FOXM1 expressing plasmid. b IL-8 levels in the CM of indicated cells were determined by ELISA. c Upper: putative FOXM1 binding sites on the promoter region of IL-8. Lower: ChIP assay of the enrichment of FOXM1 on IL-8 promoter relative to IgG in HCT116 cells overexpressing FOXM1. A random region without FHREs served as a negative control (Neg). d Tube formation assay of HUVECs in response to CM from FOXM1 overexpressing HCT116 cells with or without anti-IL-8 neutralizing antibody (5 μg/mL) treatment. Scale bar = 20 μm. e IL-8 levels in the CM of indicated cells were determined by ELISA. f Tube formation assay of HUVECs in response to CM from miR-6868-5p inhibiting HCT116 cells with or without anti-IL-8 neutralizing antibody (5 μg/mL) treatment. Scale bar = 20 μm. *p < 0.05, **p < 0.01

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