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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Hypoxia potentiates gemcitabine-induced stemness in pancreatic cancer cells through AKT/Notch1 signaling

Fig. 1

Gemcitabine promotes Notch1 activation and pancreatic cancer cell stemness. (a) PANC-1 and Patu8988 cells were treated with 0.1–500 μM gemcitabine for 24 h, and the relative survival rate was measured by the MTT assay. Western blot findings revealed (b) the representative expression levels of Bmi1, Sox2, NICD1, and Notch1 as well as (c) the changes in these levels after treatment with different concentrations of gemcitabine for 24 h. After treatment with 5 μM gemcitabine for 24 h, (d) the representative expression level of the pancreatic CSC marker CD24 as well as (e) the change in the proportion of CD24+ pancreatic CSCs were determined by FCM. (f-h) The ability of the cells to form spheres after treatment was evaluated by the sphere-forming assay in stem cell medium: (f) Representative image of sphere formation in cancer cells; (g, h) Charts showing the data on sphere number and diameter. The data are derived from three independent assays. Scale bar, 50 μm. *P < 0.05; **P < 0.01; ***P < 0.001

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