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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Hypoxia potentiates gemcitabine-induced stemness in pancreatic cancer cells through AKT/Notch1 signaling

Fig. 3

Notch1 inhibition enhances the killing effect of gemcitabine in vivo. PANC-1 cells were subcutaneously injected into the right flank of nude mice. After about 6 days, the mice were randomly divided into the control, DAPT, GEM, and GEM+DAPT groups in accordance with the protocol described in the Methods. (a) Representative tumor size at 38 days post-treatment. (b) Tumor growth curves delineated on the basis of volume measured every 4 days. (c) After treatment, the expression levels of Bmi1, Sox2, NICD1, and Notch1 were determined by Western blot analysis. (d, e) Tumor samples were digested by using collagenase I, and the change in the proportion of CD24+ pancreatic CSCs was determined by FCM. The graphs are from three independent experiments. **P < 0.01

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