Fig. 1
Cyclin G2 expression is down-regulated in gastric cancer. a Relative mRNA expression of CCNG2 in 45 paired gastric cancer and adjacent normal tissues. The quantification of CCNG2 mRNA was performed using qRT-PCR. Data are presented as log2 values in gastric cancer tissues relative to matched adjacent non-tumor tissues. b Western blot analysis for the cyclin G2 protein levels in ten gastric cancer tissues and adjacent non-cancerous gastric tissues. c Representative immunohistochemical staining with cyclin G2 antibody in gastric cancer and paired adjacent normal tissues. Six representative cases are shown. Scale bar = 100 μm. d Relative mRNA expression of CCNG2 in GES-1 and three gastric cancer cell lines (SGC-7901, AGS and MGC-803) with different differentiation statuses. CCNG2 mRNA was quantified by qRT-PCR. Data from gastric cancer cell lines are expressed relative to the GES-1 CCNG2 mRNA level (a normal gastric epithelial cell line that was chosen as a control). Results are presented as the means of values. Bars indicate standard deviation (SD; n = 3). *P < 0.05; **P < 0.01. e Comparison of the protein expression levels of cyclin G2 in three gastric cell lines and human normal gastric epithelial cell line