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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Cyclin G2 suppresses Wnt/β-catenin signaling and inhibits gastric cancer cell growth and migration through Dapper1

Fig. 3

Cyclin G2 inhibited tumor growth and metastasis in vivo. a Western blot analysis for the cyclin G2 expression in SGC-7901 cells (blank), SGC-7901 cells overexpressing cyclin G2 and GFP (NC). b–d Cyclin G2 inhibited tumor growth in the xenograft nude mice. Nude mice were subcutaneously injected with SGC-7901 cells (blank), SGC-7901 cells overexpressing cyclin G2 or control GFP (NC) by lentivirus infection. Tumor sizes were measured after sizeable tumors formation (day 14). Inoculated mice were sacrificed on day 25 and the tumors were excised for analysis (n = 5). b Macroscopic appearance of the isolated tumor. c The tumor weights in cyclin G2-overexpressing, control vector GFP-overexpressing (NC) and untransfected SGC-7901 cell (blank) groups were compared (n = 5). *P < 0.05. d Comparison of tumor volume curves in uninfected SGC-7901 cells (blank), SGC-7901 cells overexpressing cyclin G2 and GFP (NC) groups in xenograft nude mice (n = 5). e Representative immunohistochemical staining with a Ki-67 antibody of the xenograft tumor from nude mice injected with SGC-7901 negative control cells and SGC-7901 cells overexpressing cyclin G2. Bar = 100 μm. f Graph represents percentage of Ki-67-positive cells out of the total cells in each field (n = 3). **P < 0.01. g Representative immunohistochemical staining of xenograft tumors from nude mice with cyclin G2 antibody. Bar = 100 μm. h–i The effect of cyclin G2 on gastric cancer metastasis in vivo. (h) Representative images of whole lungs from nude mice inoculated with SGC-7901 cells overexpressing cyclin G2 or control GFP (NC) by lentivirus infection. (i) Statistical analysis showed a decrease in the metastatic foci of the nude mice inoculated with SGC-7901 cells overexpressing cyclin G2 compared to those overexpressing GFP. *P < 0.05. j Representative H&E stain images of Lung metastasis. Bar = 100 μm

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