Fig. 3
GRINA promoted gastric cancer proliferation in vitro and in vivo. a Relative mRNA levels of GRINA in a panel of gastric cell lines (SGC-7901, MGC-803, BGC-823, MKN45, HGC27, AGS, N87, GES-1). GRINA expression was higher in the 7 gastric cancer cell lines than in the normal immortalized gastric mucosal epithelial cell line GES-1 (N = 3). b-c Cell proliferation rates were measured using CCK8 assay in BGC-823 and AGS cells following GRINA silencing and the results showed that GRINA knockdown significantly inhibited the growth of gastric cancer cells (N = 3). d-e Cell proliferation rates were measured using CCK8 assay in HGC27 and N87 cells following GRINA overexpression and the results showed that GRINA overexpression significantly promoted the growth of gastric cancer cells (N = 3). (f-h) AGS cells stably transfected with GRINA-shRNA or vector-control were respectively injected into nude mice. Tumour weights and sizes were significantly smaller in the shRNA group than in the control group. i-k N87 cells stably transfected with GRINA-OE or vector-control were respectively injected into nude mice. Tumour weights and sizes were significantly larger in the GRINA-OE group than in the control group. l Immunofluorescence staining for Ki67 reaveled that tumor tissues in the control group showed higher positivity rate than those in the shRNA group (N = 3). Values are mean ± s.d., *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test)