Fig. 5

GRINA was transcriptionally mediated by c-Myc and promoted cell cycle transition. a-b Cell cycle assay indicated that the proportion of cells in G1/S phase was much larger in the shRNA groups than in the control groups (N = 3). c GSEA pathway analysis using TCGA database showed that GRINA expression was related to Myc targets alteration. d Pearson’s correlation coefficient revealed a positive correlation between GRINA and Myc expression in TCGA database. e A ChIP assay was performed to confirm the potential c-Myc binding site in the GRINA promoter region. In total, 10 pairs of primers were constructed according to the promoter of GRINA. f-g Knockdown of c-Myc in BGC-823 and AGS cells decreased GRINA expression significantly (N = 3). (H) CyclinD1 and CyclinE were measured by western blotting analysis after GRINA was silenced in BGC-823 and AGS cell lines (N = 3). Values are mean ± s.d., *P < 0.05, **P < 0.01 (Student’s t-test)