Fig. 1

Expression and activity of ALDH1A1 in breast cancer cells. a RT-PCR analysis of ALDH1A1 in breast cancer cells grown with 10% FBS. b Western blot analysis for ALDH1A1. β-Actin was used as loading control. Gel shown is representative of three experiments with similar results. c Variation of ALDH1A1 activity, measured by the formation of NADH in tumor cells. Breast tumor cell lysates were pretreated with CM037 (50 μM, 10 min) and absorbance at 340 nm (corresponding to NADH production) was measured. **p < 0.01 vs untreated cells