Fig. 5
ALDH1A1 affects tumor growth and vascular flow in MCF-7 tumor xenograft in athymic nude mice. MCF-7 cells (1 × 107 with 50% v/v of Matrigel) were injected s.c. in flank of athymic female nude mice. β-estradiol were injected (3 mg/kg), every 7 days i.m.. All mice were sacrificed at day 23. Tumor volumes were detected twice a week using a caliper and calculated by the formula: shortest diameter × longest diameter × thickness of the tumor in mm (n = 6 animals per group). a Tumor volume at day 23. **p < 0.01 vs Scr group. ##p < 0.01 vs ALDH1A1+ group. b Tumor mass at day 23. The tumors were weighted immediately after isolation from mice. **p < 0.01 vs Scr group. ##p < 0.01 vs ALDH1A1+ group. c Power Doppler imaging in tumors using 3D Power Doppler imaging VisualsonicVevo 2100 at the day of sacrifice. The tumor volume and percent vascularity are calculated. Red areas indicate blood flow. Images are representative of six mice per group. d Quantification of tumor vascularity (as %) by VisualsonicVevo 2100 before the sacrifice. ***p < 0.001 vs Scr group. ###p < 0.001 vs ALDH1A1+ group. e Quantification of tumor volumes by VisualsonicVevo 2100 at the day of sacrifice. ***p < 0.001 vs Scr group. ###p < 0.001 vs ALDH1A1+ group