Fig. 1

Evaluation of stemness factors on Saos2 and MG63 cell lines after VPA and DAC treatment. (a) real-time PCR for SOX2, NANOG, OCT4 and CD133 showing an increase of these genes after VPA and DAC treatments; (b) Flow cytometry analyses of increased expression of SOX2, OCT4 and NANOG in Saos2 and MG63 cells after VPA and DAC treatments; (c) up-regulation of CD133 on Saos2 and MG63 cells after VPA and DAC treatments analysed by flow cytometry. * p < 0.005, ** p < 0.001, *** p < 0.0001 compared to the untreated cells