Fig. 3
Evaluation of histone modifications, acetylation and global methylation of DNA on Saos2 and MG63 cell lines after VPA and DAC treatment. (a) Modulation of H3 trimethyl k9, H3 trimethyl k27, H3 trimethyl k4, H3 dimethyl k4 and H3 acetylation k9 by flow cytometry. VPA and DAC induced a decrease of H3 trimethyl k9 and H3 trimethyl k27, and an increase of H3 trimethyl k4, H3 dimethyl k4 and H3 acetylation k9; (b) Immunofluorescence for H3 acetylation k9 showing high levels of expression for VPA and DAC treatment; (c) Modulation of 5’methylcytidine showing a decrease of its expression after VPA and DAC treatment using flow cytometry. ** p < 0.001, *** p < 0.0001 compared to the untreated cells