Fig. 5

Analyses of stemness on shHDAC2 MG63 cell line. (a) Flow cytometry analysis of HDAC2 expression in shHDAC2 MG63#1 and 2 cells and MG63 mock after silencing; (b) Cytometric analysis of the expression of stemness factors in shHDAC2 MG63#1 and 2 cells showing a strong increase of SOX2, OCT4, NANOG and CD133 stemness markers; (c) Flow cytometry showing elevated expression of H3 acetyl k9 on shHDAC2 MG63#1 and 2 cells compared to mock cells; (d) Decrease of DNA methylation of shHDAC2 MG63#1 and 2 cells; (e) SSFE showing an increase of sarcospheres formation in shHDAC2 MG63#1 and 2 cells; (f) Soft agar assay showing increased ability to form colonies of shHDAC2 MG63#1 and 2 cells compared to those of mock cells. ** p < 0.001, *** p < 0.0001 compared to mock cells